Natalia Wörner was born in Stuttgart on September 7, 1967 and grew up in an all-women household that extended to four generations. After graduating high school, she began working as a model in Paris, Milan and New York where she eventually moved to study acting at Lee Strasberg's Actors Studio. Back in Germany, she took part in small theatre productions in Hamburg.

Wörner had her first feature parts in Sherry Horman's "Frauen sind was Wunderbares", Leo Hiemer's "Leni", and Dominik Graf's action thriller "Die Sieger" ("The Invincibles"), all in 1993. She then acted alongside Gérard Depardieu' in the international production "Die Maschine" ("La machine", 1994) followed by Horman's "Irren ist männlich" ("Father's Day", 1995).

In the following years, she worked with some of the most renowned German film and TV directors and became a well-respected actress in the process. Both her classic beauty and her acting skills made her excel in theatrical as well as in TV productions like "mammamia" (1998), Friedemann Fromm's "Zum Sterben schön" ("Good Enough To Die For", 1997), in the TV drama "Der Laden" ("The Store", 1998), in "Der Rosenmörder" (1998) or in "Das Tal der Schatten" (1999). After featuring in a supporting role in Oskar Roehler's "Suck my Dick" (2001) she focussed primarily on TV and also on theatre work.

In 2000, she was awarded the Deutscher Fernsehpreis as Best Actress for her performance in TV crime movie "Bella Block – Blinde Liebe" for which she also co-wrote the screenplay, and for her appearance in Vivian Naefe's "Frauen lügen besser".

Wörner stuck with the crime genre for the next couple of years, featuring in "Tatort" and, from 2006 to 2010, as a detective in crime series "Unter anderen Umständen". In the historical mini series adaptation of Ken Follett's "Die Säulen der Erde" ("The Pillars of the Earth", 2010) she was one of the leads.

She played a career politician confronted with her family's Nazi past in the acclaimed TV thriller "Das Kindermädchen" (2012), followed by a leading role in the successful comedy "Die Kirche bleibt im Dorf" (2012). Wörner again displayed her comic talent in the made-for-TV film "Kückückskind" (2014), in which she plays a mother who learns that her 15-year-old son was switched at birth and is actually the child of a Turkish family. After a turn in the period piece "Götz von Berlichingen" (2014, TV), Wörner starred opposite Heiner Lauterbach in the TV miniseries "Tannbach – Schicksal eines Dorfes" (2015) which chronicles life in a German village at the end of WWII.

In 2015, Natalia Wörner reprised her role as the smart owner of a country inn for the sequel "Täterätää - Die Kirche bleibt im Dorf 2".

On Christmas 2004, Natalia Wörner survived the devastating tsunami that hit Thailand. The event made her found the aid organization Tsunami Direkthilfe e.V. in 2005. Since 2006, she is also an ambassador for another relief organization, Kindernothilfe e.V.. In 2006, she married actor Robert Seeliger but got divorced two years later. Natalia Wörner lives in Berlin with her son.