Luise Helm was born on March 6, 1983 in East Berlin (then German Democratic Republic) as the daughter of actor Gunnar Helm. She played her first television role at the age of ten, in an episode of the series "Ein Bayer auf Rügen" (1994). Bernd Böhlich cast her in the title role of a child with cancer in "Natascha - Wettlauf mit dem Tod" (1996); in Carlo Rola's adaptation of the novel "Der stille Herr Genardy" (1997), she was the daughter of Iris Berben's main character.   

Helm achieved greater fame in 2000 with her leading role in the teenage comedy "Harte Jungs" alongside Tobias Schenke and Axel Stein. She had significant roles in the drama "Königskinder" (2003), as a teenager who gets pregnant unintentionally, and in the ensemble of the award-winning drama "12 heißt: Ich liebe dich" (2007). On the big screen, she was seen in a leading role in Lars Jessen's "Am Tag als Bobby Ewing starb" (2005), as the mayor's daughter and an opponent of nuclear power plants who is ready to use violence.   

Helm appeared mainly in various television series. She was part of the ensemble of the series "Outriders - Abenteuer Australien" (2001) and played the daughter of Imogen Kogge's investigator in the RBB episodes of the crime series "Polizeiruf 110" between 2002 and 2008. In the web series "Straight Family" (2018), she had a lead role as a young woman who just had her lesbian coming out. Furthermore Helm appeared in guest roles in various series, including "Das Traumschiff" (2005), "Kommissar Stolberg" (2009), "SOKO Stuttgart" (2010), "Letzte Spur Berlin" (2012) and "In aller Freundschaft" (2016). 

Helm played a leading feature film role as a budding social media star in the mockumentary "Die Essenz des Guten" (2012). She was then an associate producer on RP Kahl's "A Thought of Ecstasy" ("Ecstasy", DE/US/CH 2017).  After a small appearance as a nurse in Julie Delpy's "My Zoe" (FR/DE/PT 2019), Helm was seen in a leading role in RP Kahl's "Als Susan Sontag im Publikum saß" ("When Susan Sontag Was Sitting in the Audience", 2021), in which she played the US author and LGBT activist Jill Johnston. 

In addition to screen acting, Luise Helm is also a voice actress. Among others, she has been the German voice of Scarlett Johansson since 2005 and of Megan Fox and Sienna Miller since 2007. She has also worked on numerous audio books since 2011.