Matthias Weidenhöfer was born in Achim on April 16, 1985. From 2007 until 2010 he studied acting at the Hochschule für Film und Fernsehen in Potsdam. During that time, he and the other members of the school's acting troupe received an ensemble award at the Theatertreffen Deutschsprachiger Schauspielstudierender in Zurich.

Following his graduation, Weidenhöfer performed at several theatres including the Sophiensaele in Berlin and the Schauspielhaus Zurich. In 2010, he made his screen debut as a scheming landlord in "Krankheit der Jugend". The following year, he was part of the ensemble cast of Christian Schwochow's drama "Die Unsichtbare" ("Cracks in the Shell"). On TV, Weidenhöfer guest-starred in shows like "Ein starkes Team", "Wilsberg" and "Küstenwache", as well as in several entries of the "Tatort" series. He then produced Isabell Šuba's mockumentary "Männer zeigen Filme & Frauen ihre Brüste" ("Men Show Movies & Women Their Breasts", 2013), which featured him also as the male lead: He plays a fledgling producer who accompanies a young female director to the Cannes IFF in order to present a short film. Šuba's award-winning feature screened at several festivals and was released theatrically in summer 2014.